Optimization of low-cost medium for very high gravity ethanol fermentations by ...

• Author(s): Pereira, Francisco B. ; Guimarães, Pedro M. R. ; Teixeira, J. A. ; Domingues, Lucília
• Date: 2010
• Persistent ID: http://hdl.handle.net/1822/11466
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Very high gravity fermentation; Corn steep liquor; Ethanol; Response surface methodology; Saccharomyces cerevisiae
• Document Type: Article
• Language: English

Description

Statistical experimental designs were used to develop a medium based on corn steep liquor (CSL) and other low-cost nutrient sources for high-performance very high gravity (VHG) ethanol fermentations by Saccharomyces cerevisiae. The critical nutrients were initially selected according to a Plackett–Burman design and the optimized medium composition (44.3 g/L CSL; 2.3 g/L urea; 3.8 g/L MgSO4·7H2O; 0.03 g/L CuSO4·5H2O) for maximum ethanol production by the laboratory strain CEN.PK 113-7D was obtained by response surface methodology, based on a three-level four-factor Box-Behnken design. The optimization process resulted in significantly enhanced final ethanol titre, productivity and yeast viability in batch VHG fermentations (up to 330 g/L glucose) with CEN.PK113-7D and with industrial strain PE-2, which is used for bio-ethanol production in Brazil. Strain PE-2 was able to produce 18.6 ± 0.5% (v/v) ethanol with a corresponding productivity of 2.4 ± 0.1 g/L/h. This study provides valuable insights into cost-effective nutritional supplementation of industrial fuel ethanol VHG fermentations.