Studies on the interaction of the carbohydrate binding module 3 from the Clostr...

• Author(s): Machado, João ; Araújo, Adriano ; Pinto, João Ricardo ; Gama, F. M.
• Date: 2009
• Persistent ID: http://hdl.handle.net/1822/10417
• Origin: RepositóriUM - Universidade do Minho
• Subject(s): Cellulose; CBM3; Clostridium thermocellum; Adsorption; Pulp drainability
• Document Type: Article
• Language: English

Description

The adsorption of a carbohydrate binding module (CBM3) from the Clostridium thermocellum scaffolding protein (CipA) to cellulose was analysed in this work. The effect of CBM-PEG on the drainability of E. globulus and P. sylvestris pulps and on the physical properties of the respective papersheets was also studied. The CBM binding to cellulose is often described as “irreversible”, but this classification does not fully characterize this interaction. Indeed, the results obtained demonstrate that, although the adsorption on cellulose is rather stable, CBM inter-fibre mobility may be observed. The results also showed that the CBM-PEG conjugate improves the drainability of E. globulus and P. sylvestris pulps without affecting the physical properties of the papersheets.