Mycobacterium avium subsp. paratuberculosis infection in slaughtered domestic p...

• Author(s): Miranda, Carla ; Matos, Manuela ; Pires, Maria dos Anjos ; Ribeiro, Paulo ; Álvares, Sofia ; Vieira-Pinto, Madalena ; Coelho, Ana Cláudia
• Date: 2011
• Persistent ID: http://hdl.handle.net/10348/2940
• Origin: Repositório da UTAD
• Subject(s): Mycobacterium avium subsp. paratuberculosis; Swine; PCR
• Document Type: Article
• Language: English

Description

Herein, we report the occurrence of Mycobacterium avium subsp. paratuberculosis (Map), in four domestic pigs slaughtered for consumption that revealed compatible lesions of Mycobacterium spp. Infection in submaxillary and in mesenteric lymph nodes during the post mortem examination was observed. Histopathologic evaluation revealed granulomatous lesions mostly consisted by central necrosis, associated with inflammatory infiltration. Two cases presented dystrophic calcification. Acid-fast bacilli were observed in all cases. Three different PCR assays were carried out to confirm the presence of Map. In swine there are few published cases of Map infection and to the best of our knowledge, this is the first report of molecular detection of Map directly from pig tissues.