Phylogenetic identification of Aeromonas simiae from a pig, first isolate since...

• Autor(es): Fontes, M.C. ; Saavedra, M.J. ; Martins, C. ; Monera, A. ; Martínez-Murcia, A.
• Data: 2010
• Identificador Persistente: http://hdl.handle.net/10348/2781
• Origem: Repositório da UTAD
• Assunto(s): Aeromonas simiae; phylogeny; 16S rDNA; gyrB; rpoD
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

The species Aeromonas simiae was first described on the basis of two strains (CIP 107798T and CIP 107797) isolated from faeces of healthy monkeys (M. fascicularis) from Mauritius, which were kept in quarantine in the Centre for Primatology, Louis Pasteur University, Strasbourg, France. In the present study, during a survey to determine the prevalence of Aeromonas at three different pig slaughterhouses at north of Portugal, a single strain MDC2374 (out of 703 isolates) was identified as Aeromonas simiae on the basis of 16S rDNA, gyrB and rpoD sequencing, confirming that traditional biochemical approaches are not resolute to identify rarely isolated Aeromonas species. To our knowledge, this is the first time since species description that A. simiae is newly isolated and identified. The MDC work has been supported by Grant IMIDTA/2007/68 from IMPIVA, Generalitat Valenciana, Spain. M.J. Saavedra and M.C. Fontes were the recipients of grants SFRH/BSAB/774/2008 and SFRH/BD/25415/2005, respectively, from theFundação para a Ciência e Tecnologia. Part of this work was also supported by funds from the European Commission for the HEALTHY WATER Project (FOOD-CT-2006-036306).