Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissue...

• Author(s): Coelho, Ana Cláudia ; Pinto, Maria de Lurdes ; Miranda, Alice ; Coelho, Adosinda ; Pires, Maria dos Anjos ; Matos, Manuela
• Date: 2010
• Persistent ID: http://hdl.handle.net/10348/2697
• Origin: Repositório da UTAD
• Subject(s): Mycobacterium avium subsp. paratuberculosis; PCR; Tissues; Ziehl-Neelsen
• Document Type: Article
• Language: English

Description

Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.