Lignin transformation by a versatile peroxidase from a novel bjerkandera sp. st...

• Author(s): Moreira, Patrícia Raquel ; Almeida-Vara, Elsa ; Malcata, Francisco Xavier ; Duarte, José Cardoso
• Date: 2007
• Persistent ID: http://hdl.handle.net/10400.14/2834
• Origin: Veritati - Repositório Institucional da Universidade Católica Portuguesa
• Subject(s): Enzyme; Wood material; Gel filtration chromatography
• Document Type: Article
• Language: English

Description

A versatile peroxidase, purified from a novel strain of Bjerkandera sp. (B33/3), was tested for its reactivity on a lignin fraction obtained from straw pulping. The effects of such processing parameters as reaction time, pH, and lignin:enzyme ratio were evaluated. Gel filtration chromatography was employed to characterise the molecular mass distribution of the lignin fragments produced by the enzyme-mediated reaction. Our results have shown that such a versatile peroxidase can directly bring about transformations of lignin, even in the absence of external mediators.