Mass spectrometric analysis of epimeric 3α/3β functionalized steroids.

• Autor(es): Ramos, Susana Sofia ; Ramalho, Luís ; Costa, Mafalda ; Teixeira, Adriano ; Almeida, Paulo ; Costa, Maria do Céu
• Data: 2007
• Identificador Persistente: http://hdl.handle.net/10400.9/695
• Origem: Repositório do LNEG
• Assunto(s): APCI; EI; Tandem MS; Epimer discrimination
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Tandem mass spectrometry (MS/MS) using an atmospheric pressure chemical ionization (APCI) source in positive mode (+) reveals different intensity ratios of two fragment peaks of the epimeric cholestane derivatives synthesized from 3{460}- or 3β-cholestanol. The investigation of the fragmentation pattern obtained from their full scan and/or collision induced dissociation (CID) spectra in the case of the cholestane skeleton together with the respective electronic ionization (EI) spectra supports a distinction of the epimers.