Identification of a 0.4 Kb deletion region in 10q26 associated with endometrial...

• Author(s): Alves, Margarida ; Carreira, Isabel ; Liberato, Paulo ; Ramos, Sância ; Mafra, Manuela ; Inverno, Alexandra S. ; Maia, Ana T. ; Martins, Ana Paula ; Brito, Miguel ; Monteiro, Carolino
• Date: 2010
• Persistent ID: http://hdl.handle.net/10400.21/3070
• Origin: Repositório Científico do Instituto Politécnico de Lisboa
• Subject(s): Carcinoma; Chromosome deletion; Chromosome mapping; DNA mutational analysis; DNA replication; Endometrial neoplasms; Genetic linkage; Loss of heterozygosity; Matched-Pair analysis; Microsatellite instability
• Document Type: Article
• Language: English

Description

We have identified an allelic deletion common region in the q26 region of chromosome 10 in endometrial carcinomas, which has been reported previously as a potential target of genetic alterations related to this neoplasia. An allelotyping analysis of 19 pairs of tumoral and non-tumoral samples was accomplished using seven microsatellite polymorphic markers mapping in the 10q26 chromosomal region. Loss of heterozygosity for one or more loci was detected in 29% of the endometrial carcinoma samples. The observed pattern of loss enabled the identification of a 3.5 Mb common deleted region located between the D10S587 and D10S186 markers. An additional result from an endometrial sample with evidence of a RER phenotype may suggest a more centromeric region of loss within the above-mentioned interval. This 401.84 Kb interval flanked by the D10S587 and D10S216 markers may be a plausible location for a putative suppressor gene involved in early stage endometrial carcinogenesis.