Cox-2 inhibition with nutraceuticals: a new therapeutic approach against Helico...

• Autor(es): Santos, António Mário ; Oleastro, Mónica ; Lopes, Teresa ; Pereira, Teresa ; Seixas, Elsa ; Chaves, Paula ; Machado, Jorge ; Guerreiro, António Sousa
• Data: 2012
• Identificador Persistente: http://hdl.handle.net/10400.18/1327
• Origem: Repositório Científico do Instituto Nacional de Saúde
• Assunto(s): Helicobacter pylori Infection; COX)-2; Nutraceuticals
• Tipo de Documento: Documento de conferência
• Idioma: Inglês

Descrição

Abstract no.: P3.7 publicado em: Helicobacter 2012;17(Suppl.1):98 Accumulated evidence in humans and animals shows that H. pylori up-regulate the expression of cyclooxygenase (COX)-2 both at mRNA and protein levels which might be one of the mechanisms leading to several gastric diseases. Aim: To study the expression of COX-2 on mice gastric mucosa during long-term treatment with two nutraceuticals: curcumin and synbiotic 2000 on H. pylori experimental chronic infection. Materials and Methods: We infected 45 C57BL/6 mice with SS1 – H. pylori strain. After infection confirmation by 13C-urea breath test mice where then treated with either PBS, curcumin (10 mg/mouse) or Synbiotic 2000 (50 mg/ mouse), three times per week. Five mice from each treatment group were euthanized at week 6, 18 and 27. Gastric samples were removed for COX-2 immunohistochemistry analysis. Results: All the 45 mice were Hp positive by 13C-urea breath test and immunohistochemistry. In the PBS group the production of COX-2 was significantly up-regulated at week 6 (area of positive immunostaining 393–544 · 103 pixels), 18 (area of positive immunostaining 242–614 · 103 pixels) and 27 week (area of positive immunostaining 129–175 · 103 pixels). The treatment with either curcumin or synbiotic significantly decreased the expression of COX-2 at all time points. Conclusions: These results suggest the therapeutic usefulness of both nutraceuticals on COX-2 inhibition during chronic experimental mice H. pylori infection. The supplementation of diet in humans with curcumin or Synbiotic 2000 may be a novel therapeutic approach against gastric inflammation induced by Hp infection.