13C MRS: An outstanding tool for metabolic studies

• Autor(es): Rodrigues, Tiago B. ; Cerdán, Sebastián
• Data: 2005
• Identificador Persistente: http://hdl.handle.net/10316/8123
• Origem: Estudo Geral - Universidade de Coimbra
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

We provide an overview of 13C magnetic resonance spectroscopy methods and their applications as an established metabolic tool with an emphasis on the use of high-resolution 13C magnetic resonance spectroscopy and 13C isotopomer analysis. Topics addressed include general properties of the 13C magnetic resonance spectroscopy spectrum; different 13C magnetic resonance spectroscopy acquisition protocols; determination of fractional 13C enrichment, kinetic, or steady state measurements of metabolic flux; 13C isotopomer analysis approaches; 13C(2H) magnetic resonance spectroscopy methodologies; and in vivo 13C magnetic resonance spectroscopy. Some illustrative applications are described. © 2005 Wiley Periodicals, Inc. Concepts Magn Reson Part A 27A: 1-16, 2005. http://dx.doi.org/10.1002/cmr.a.20039