Author(s):
Ramos, F. 
; Matos, A. ; Oliveira, A. ; Silveira, M. da
Date: 1999
Persistent ID: http://hdl.handle.net/10316/7939
Origin: Estudo Geral - Universidade de Coimbra
Document details
Diphasic dialysis extraction technique for clenbuterol determination in bovine ...
Description
Summary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria. http://dx.doi.org/10.1007/BF02493629
Summary A method is described for the extraction of clebuterol from bovine retina samples by diphasic dialysis using gas chromatography—mass spectrometry (GC-MS) as the procedure of determination. Following chemical digestion of retinal tissue in alkaline medium at 80C, clenbuterol is extracted by diphasic dialysis using diethyl ether as solvent at 37C, for 4 h at 150 rpm. Diethyl ether is evaporated to dryness and clenbuterol determined by GC-MS after butylboronic acid (BBA) derivatization. A detection limit of 2.5 ng g-1, repeatability of 9.0% and reproducibility of 22.9%, with recoveries always >86% (n=5), are achieved as validation criteria. http://dx.doi.org/10.1007/BF02493629
Document Type
Article
Language English
Language English
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