Author(s):
Santos, Maria 
; Pereira, Emilia ; Carvaho, Arsélio
Date: 1999
Persistent ID: http://hdl.handle.net/10316/7900
Origin: Estudo Geral - Universidade de Coimbra
Document details
Stimulation of Immunoreactive Insulin Release by Glucose in Rat Brain Synaptosomes
Description
The effect of glucose on the release of immunoreactive insulin (IRI) in synaptosomes isolated from rat brain was studied. In the absence of glucose synaptosomes release about 4% (0.77 µIU/mg protein) of total content. Glucose increases significantly the IRI released by synaptosomes. Addition of the glycolytic inhibitor iodoacetic acid (IAA), decreased the glucose-induced release of IRI by about 50%, suggesting that glucose metabolism is involved. The observation that glucose provides a concentration related signal for IRI release indicates that this synaptosomal preparation may be useful as a model for research on the mechanism of insulin release in brain. http://dx.doi.org/10.1023/A:1020971812098
The effect of glucose on the release of immunoreactive insulin (IRI) in synaptosomes isolated from rat brain was studied. In the absence of glucose synaptosomes release about 4% (0.77 µIU/mg protein) of total content. Glucose increases significantly the IRI released by synaptosomes. Addition of the glycolytic inhibitor iodoacetic acid (IAA), decreased the glucose-induced release of IRI by about 50%, suggesting that glucose metabolism is involved. The observation that glucose provides a concentration related signal for IRI release indicates that this synaptosomal preparation may be useful as a model for research on the mechanism of insulin release in brain. http://dx.doi.org/10.1023/A:1020971812098
Document Type
Article
Language English
Language English
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