Determination of ochratoxin A in organic and non-organic cereals and cereal pro...

• Autor(es): Juan, C. ; Moltó, J. C. ; Lino, C. M. ; Mañes, J.
• Data: 2008
• Identificador Persistente: http://hdl.handle.net/10316/5841
• Origem: Estudo Geral - Universidade de Coimbra
• Assunto(s): Organic; Non-organic; Ochratoxin A; Portugal
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

The objective of this work was to know the occurrence of OTA in organic and non-organic cereals and cereal products from Spain and Portugal. A method based on extraction with matrix solid phase dispersion (MSPD) using octylsilica (C8) followed by liquid chromatography coupled with fluorescence detection (LC-FD) was used to determine OTA from the selected samples. Recoveries of OTA from the studied samples spiked at 10 ng/g level ranged from 78% to 89% with a standard deviation of 3.66. The limits of detection and quantification of this method were 0.05 and 0.19 ng/g, respectively. Furthermore, LC-FD after OTA methylation was used to confirm the identity of OTA in all positive samples. This procedure was applied to 83 organic and non-organic samples including rice, wheat, barley, rye, oats and maize from Spain and Portugal. OTA was detected in 22% of the samples, with concentrations ranging from 0.20 to 27.10 ng/g. From the total OTA contaminated samples (n = 18), 72% were organic cereal and 28% were non-organic cereal samples, with mean concentrations of 1.64 and 0.05 ng/g, respectively. The 66% and 34% of contaminated samples were from Spain and Portugal, respectively, with mean concentrations of 0.93 and 0.64 ng/g for each country. Six contaminated samples exceeded the maximum limits (ML) for OTA fixed by European Commission Regulation (5 [mu]g/kg), among them three were from Spain and three from Portugal. http://www.sciencedirect.com/science/article/B6T6R-4PDSBTJ-2/1/a9837f039ed5fc5fbbb1b924528f714f