Analytical method for the determination of strychnine in tissues by gas chromat...

• Autor(es): Marques, E. P. ; Gil, F. ; Proença, P. ; Monsanto, P. ; Oliveira, M. F. ; Castanheira, A. ; Vieira, D. N.
• Data: 2000
• Identificador Persistente: http://hdl.handle.net/10316/4838
• Origem: Estudo Geral - Universidade de Coimbra
• Assunto(s): Strychnine; Rodenticide; Alkaloid
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

This paper describes an analytical method for strychnine determination in biological samples by gas chromatography/mass spectrometry and their application in the investigation of two cases involving strychnine ingestion: A fatal case and a clinical one. The strychnine is isolated from biological samples using a liquid-liquid extraction procedure. The clean-up procedure is performed using an acid solution. Papaverine is used as internal standard in the quantification of strychnine. In the analysed specimens, the limits of quantification were 0.1 [mu]g/ml or 0.1 [mu]g/g. The recovery rate ranged from 75.0% to 98.7% and the coefficients of variation ranged from 4.8% to 10.5%. http://www.sciencedirect.com/science/article/B6T6W-408KCFB-8/1/f8bbeefad9e20909aef50467f67a2e84