Detalhes do Documento

Synthesis and Cytotoxic Profile of 3,4-Methylenedioxymethamphetamine (“Ecstasy”...

Autor(es): Milhazes, Nuno cv logo 1 ; Cunha-Oliveira, Teresa cv logo 2 ; Martins, Pedro cv logo 3 ; Garrido, Jorge cv logo 4 ; Oliveira, Catarina cv logo 5 ; Rego, A. Cristina cv logo 6 ; Borges, Fernanda cv logo 7

Data: 2006

Identificador Persistente: http://hdl.handle.net/10316/12872

Origem: Estudo Geral - Universidade de Coimbra


Descrição
The toxicological and redox profiles of MDMA and its major metabolites (MDA, α-methyldopamine, N-methyl-α-methyldopamine, 6-hydroxy-α-methyldopamine, 3-methoxy-α-methyldopamine) were studied to establish a structure-toxicity relationship and determine their individual contribution to cell death induction by apoptosis and/or necrosis. The results of the comparative toxicity study, using undifferentiated PC12 cells, strongly suggest that the metabolites possessing a catecholic group are more toxic to the cells than MDMA and metabolites with at least one protected phenolic group. Redox studies reveal that an oxidative mechanism seems to play an important role in metabolite cytotoxicity. Nuclear features of apoptosis and/or necrosis show that most of the metabolites, particularly N-methyl-α-methyldopamine, induce cell death by apoptosis, largely accompanied by necrotic features. No significant differences were found between MDMA and the metabolites, concerning overall characteristics of cell death. These results may be useful to ascertain the contribution of metabolism in MDMA neurotoxicity molecular mechanisms
Tipo de Documento Artigo
Idioma Inglês
delicious logo  facebook logo  linkedin logo  twitter logo 
degois logo
mendeley logo

Documentos Relacionados



    Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência Programa Operacional da Sociedade do Conhecimento União Europeia