Protein kinase C isoform specificity of cholinergic potentiation of glucose-ind...

• Autor(es): Baldeiras, Inês E. ; Santos, Rosa M. ; Rosário, Luís M.
• Data: 2006
• Identificador Persistente: http://hdl.handle.net/10316/12862
• Origem: Estudo Geral - Universidade de Coimbra
• Assunto(s): Cytosolic free Ca2+ concentration; 5-HT amperometry; Islet of Langerhans; Protein kinase C; Pulsatile insulin release; Thymeleatoxin
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 \u03bcM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion