Efficient Chemoenzymatic Synthesis, Cytotoxic Evaluation, and SAR of Epoxysterols

• Author(s): Carvalho, João F. S. ; Silva, M. Manuel Cruz ; Moreira, João N. ; Simões, Sérgio ; Melo, M. Luísa Sá e
• Date: 2009
• Persistent ID: http://hdl.handle.net/10316/10535
• Origin: Estudo Geral - Universidade de Coimbra
• Document Type: Article
• Language: English

Description

A library of diastereomerically pure epoxysterols, prepared by combining chemical and enzymatic methodologies, was evaluated for cytotoxicity toward human cancer and noncancer cell lines. Unsaturated steroids were oxidized by magnesium bis(monoperoxyphthalate) hexahydrate in acetonitrile, and the resulting epimeric epoxides were enzymatically separated using Novozym 435 or lipase AY. Some of the synthesized epoxysterols have potent cytotoxicity and higher activity on cancer cell lines HT29 and LAMA-84. http://dx.doi.org/10.1021/jm9003973