Author(s):
Fonseca, L. 
; Cardoso, J. M. S. ; Moron-Lopez, J. ; Abrantes, I. M. O.
Date: 2014
Persistent ID: http://hdl.handle.net/10316/25779
Origin: Estudo Geral - Universidade de Coimbra
Document details
Bursaphelenchus fungivorus from Pinus pinaster bark in Portugal
Description
Bursaphelenchus fungivorus is reported for the first time in Portugal, identified as associated with Pinus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA. Intraisolate genetic variability was detected among ITS sequences of the Portuguese B. fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of Bursaphelenchus species, revealed that the Portuguese B. fungivorus isolate clusters with other B. fungivorus isolates, forming a separate group close to B. seani highlighting a molecular proximity of these two species. This research was partially supported by FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE) and national funds through FCT (Fundação para a Ciência e a Tecnologia) under the project FCOMP-01-0124-FEDER-008794 (FCT PTDC/AGRCFL/ 098916/2008). J. M. S. Cardoso is funded by a post-doctoral fellowship SFRH/BPD/73724/2010 financed by QREN – POPH – Typology 4.1 – cofinanced by MES national funding and The European Social Fund.
Bursaphelenchus fungivorus is reported for the first time in Portugal, identified as associated with Pinus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer (ITS) regions of ribosomal DNA. Intraisolate genetic variability was detected among ITS sequences of the Portuguese B. fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of Bursaphelenchus species, revealed that the Portuguese B. fungivorus isolate clusters with other B. fungivorus isolates, forming a separate group close to B. seani highlighting a molecular proximity of these two species. This research was partially supported by FEDER funds through the Programa Operacional Factores de Competitividade (COMPETE) and national funds through FCT (Fundação para a Ciência e a Tecnologia) under the project FCOMP-01-0124-FEDER-008794 (FCT PTDC/AGRCFL/ 098916/2008). J. M. S. Cardoso is funded by a post-doctoral fellowship SFRH/BPD/73724/2010 financed by QREN – POPH – Typology 4.1 – cofinanced by MES national funding and The European Social Fund.
Document Type
Article
Language English
Language English
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