Disposition of [U-2H7]glucose into hepatic glycogen in rat and in seabass

• Autor(es): Martins, Fátima O. ; Rito, João ; Jarak, Ivana ; Viegas, Ivan ; Pardal, M. A. ; Macedo, M. Paula ; Jones, John G.
• Data: 2013
• Identificador Persistente: http://hdl.handle.net/10316/25738
• Origem: Estudo Geral - Universidade de Coimbra
• Assunto(s): Direct pathway; Transaldolase; Glycogenesis; Gluconeogenesis; Seabass; Rat
• Tipo de Documento: Artigo
• Idioma: Inglês

Descrição

The stimulation of hepatic glycogenesis is a ubiquitous response to a glucose challenge and quantifying its contribution to glucose uptake informs its role in restoring euglycemia. Glycogenesis can be quantified with labeled water provided that exchange of glucose-6-phosphate hydrogen 2 (G6P-H2) and body water via glucose-6-phosphate isomerase, and exchange of positions 4, 5 and 6 hydrogens (G6P-H456) via transaldolase, are known. These exchanges were quantified in 24-h fasted rats (Rattus norvegicus; n=6) and 21-day fasted seabass (Dicentrarchus labrax; n = 8) by administration of a glucose load (2000 mg·kg−1) enriched with [U-2H7]glucose and by quantifying hepatic glycogen 2H-enrichments after 2 h (rats) and 48 h (seabass). Direct pathway contributions of the glucose load to glycogenesis were also estimated. G6P-H2 and body water exchange was 61 ± 1% for rat and 47 ± 3% for seabass. Transaldolase-mediated exchange of G6P-H456 was 5 ± 1% for rat and 10 ± 1% for seabass. Conversion of the glucose load to hepatic glycogen was significant in seabass (249 ± 54 mg·kg−1) but negligible in rats (12 ± 1 mg·kg−1). Preload plasma glucose levels were similar for seabass and rats (3.3 ± 0.7 and 4.4 ± 0.1 mmol·L−1, respectively) but post-load plasma glucose was significantly higher in seabass compared to rats (14.6 ± 1.8 versus 5.8 ± 0.3 mmol·L−1, p b 0.01). In conclusion, G6P-H2 and body water exchange is incomplete for both species and has to be accounted for in estimating hepatic glycogen synthesis and direct pathway activities with labeled water tracers. Transaldolase-mediated exchange is insignificant. Hepatic direct pathway glycogenesis plays a prominent role in seabass glucose load disposal, but a negligible role in the rat. The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT) in the form of a Ph.D. Fellowship to F.O.M.: SFRH/ BD/51194/2010, and Research Grants to J.G.J.: PTDC/EBB-BIO/ 098111/2008 & PTDC/SAU-MET/111398/2009. The NMR spectrometer is part of the National NMR Network and was purchased in the framework of the National Programme for Scientific re-equipment, contract REDE/1517/RMN/2005, with funds from POCI 2010 (FEDER) and FCT.