Autor(es):
Capela-Pires, J 
; Ferreira, Rui ; Alves-Pereira, I
Data: 2012
Identificador Persistente: http://hdl.handle.net/10174/7406
Origem: Repositório Científico da Universidade de Évora
Assunto(s): TiO2-NP; GR; GPx
Descrição
Nanoparticles of titanium dioxide, widely used as pigments or cosmetics, are usually found in molecular size < 100 nm. Sometimes, their poor thermal stability and large surface area can be correlated with their reactivity to cause changes in gene expression that may be used as biomarkers of exposure and cytotoxicity. Although the nanomaterials may be useful in medicine, their exposition to air or ultraviolet radiation for time periods as short as 30 min, makes them redox actives agents that induce lipid peroxidation and glutathione depletion, cytotoxic responses, that cause changes in permeability or conductivity of biological membranes. The main purpose of this work was to compare the response of S. cerevisiae UE-ME3, a wine wild-type strain and BY4741, an invitrogen strain, to TiO2-NP exposition in heat-shock conditions. Cells growing at mid exponential phase in liquid YEPD medium with 2 % (w/v) glucose, at 28 ºC, were dark-exposed to heat-shock of 40 ºC and 0.1 or 1.0 µg/mL of TiO2-NP, during 200 min, prepared by sonication, at same temperature conditions. Samples of each treatment were used to obtain the post-12000 g supernatant for determination of dry weight, proteins, and glutathione contents as well as GR and GPx activities. The results show that heat-shock (28/40ºC) caused a significant decrease in proteins and dry weight of BY4741 and UE-ME3 strains, response that was accompanied by an increase in glutathione content and GSH/GSSG ratio, more pronounced in the BY4741 strain as well as a decrease in GR activity, case more pronounced in wild-type strain. Exposure to titanium dioxide nanoparticles (0.1 and 1 µg/ml) in heat-shock conditions induced a significant decrease in biomass, of UE-ME3 strain followed by a significant decrease of GR and GPx activities. The best survival of BY4741 cells to TiO2-NP seems related with an increase of GR activity, directly dependent of exposure level (r = 0.9996). The modeling of GR activity seems critical to regulate the growth inhibition of proliferating cells by heat-shock and titanium dioxide nanoparticles.
