Autor(es):
Penas, Ana Catarina 
; Correia, Petra ; Bravo, Maria Antónia ; Mota, Manuel ; Tenreiro, Rogério
Data: 2004
Identificador Persistente: http://hdl.handle.net/10174/6435
Origem: Repositório Científico da Universidade de Évora
Assunto(s): diagnostics; identification; ITS-RFLP; morphology; Pinus pinaster,; survey.
Descrição
Summary – Species of Bursaphelenchus associated with maritime pine, Pinus pinaster, from Portugal – within and outside the
quarantine restricted demarcated zone of B. xylophilus – are described and characterised both morphologically (LM and SEM) and with
the use of molecular biology (ITS-RFLP).A new staining method for spicules is proposed. Species include B. hellenicus, B. hylobianum,
B. leoni, B. pinophilus, B. sexdentati, B. tusciae, B. teratospicularis, B. xylophilus and Bursaphelenchus sp. 1. Bursaphelenchus
hylobianum was collected from the insect Hylobius sp. The most frequent species in the demarcated zone, besides B. xylophilus, was
Bursaphelenchus sp. 1. Morphological characterisation is compared with the original descriptions and discussed. The differentiation
between B. pinophilus and B. sexdentati is not clear in the literature and is discussed. Since differentiation of B. xylophilus (mucronate
form) from B. mucronatus, and B. pinophilus from B. sexdentati, as well as their juvenile forms, is almost im possible on the basis
of morphological features, a molecular approach based on ITS-RFLPs was used. Ribosomal DNA containing the 5.8S gene, the
internal transcribed spacer region 1 and 2, and partial regions of 18S and 28S gene were amplified by PCR. Restriction profiles
of the amplified products generated species-specific differences, leading to the unambiguous identification of isolates belonging to
B. xylophilus, B. mucronatus, B. sexdentati, B. tusciae and B. hylobianum.
