Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Ver...

• Author(s): Živković, Jelena ; Barreira, João C.M. ; Stojković, Dejan ; Cebovic, Tatjana ; Santos-Buelga, Celestino ; Maksimović, Zoran ; Ferreira, Isabel C.F.R.
• Date: 2014
• Persistent ID: http://hdl.handle.net/10198/10144
• Origin: Biblioteca Digital do IPB
• Subject(s): Veronica urticifolia; Phenolic profile; HPLC-DAD/ESI-MS; Antibacterial; Antimutagenicity; Ehrlich carcinoma
• Document Type: Article
• Language: English

Description

This study was designed to characterize the phenolic profile and evaluate the antibacterial, antimutagenic and antitumor activities of Veronica urticifolia Jacq. methanolic extract. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumor activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumor cell viability, while ascites volume and tumor cell count became slightly decreased, but not to a statistically significant extent. Above results indicate that V. urticifolia deserves further research into chemoprevention.