Cyclooxygenase-2 (COX-2) expression by in vitro produced bovine embryos. Prelim...

• Author(s): Marques, C.C. ; Horta, A.E. ; Vasques, M.I. ; Baptista, M.C. ; Becker, J.D. ; Pimenta, J. ; Pereira, R.M.
• Date: 2005
• Persistent ID: http://hdl.handle.net/10400.7/70
• Origin: ARCA - Access to Research and Communication Annals
• Subject(s): COX-2; embryos; in vitro; bovine; embriões; bovinos
• Document Type: Article
• Language: Portuguese

Description

Expression of the two isoforms of cyclooxygenase enzyme, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), by in vitro produced bovine embryos was examined. Cumulus-oocyte complexes were recovered from ovaries of slaughtered animals and subsequently in vitro matured and in vitro fertilized. Presumptive zygotes were cultured in serum containing medium (TCM 199+10% bovine superovulated oestrus serum) on a granulosa cell monolayer for 12 days.